Two-step derivatization for determination of sugar phosphates in plants by combined reversed phase chromatography/tandem mass spectrometry.
Identifieur interne : 000648 ( Main/Exploration ); précédent : 000647; suivant : 000649Two-step derivatization for determination of sugar phosphates in plants by combined reversed phase chromatography/tandem mass spectrometry.
Auteurs : Umut Rende [Suède] ; Totte Niittyl [Suède] ; Thomas Moritz [Suède, Danemark]Source :
- Plant methods [ 1746-4811 ] ; 2019.
Abstract
Background
Sugar phosphates are important intermediates of central carbon metabolism in biological systems, with roles in glycolysis, the pentose-phosphate pathway, tricarboxylic acid (TCA) cycle, and many other biosynthesis pathways. Understanding central carbon metabolism requires a simple, robust and comprehensive analytical method. However, sugar phosphates are notoriously difficult to analyze by traditional reversed phase liquid chromatography.
Results
Here, we show a two-step derivatization of sugar phosphates by methoxylamine and propionic acid anhydride after chloroform/methanol (3:7) extraction from
Conclusion
The results show the reliability of combined reversed phase liquid chromatography-tandem mass spectrometry for sugar phosphate analysis and demonstrate the presence of two unknown sugar phosphates in
DOI: 10.1186/s13007-019-0514-9
PubMed: 31719834
PubMed Central: PMC6836659
Affiliations:
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wicri:level="1"><nlm:affiliation>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå</wicri:regionArea><wicri:noRegion>901 83 Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Moritz, Thomas" sort="Moritz, Thomas" uniqKey="Moritz T" first="Thomas" last="Moritz">Thomas Moritz</name><affiliation wicri:level="1"><nlm:affiliation>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 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Umeå</wicri:regionArea><wicri:noRegion>901 83 Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Niittyl, Totte" sort="Niittyl, Totte" uniqKey="Niittyl T" first="Totte" last="Niittyl">Totte Niittyl</name><affiliation wicri:level="1"><nlm:affiliation>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå</wicri:regionArea><wicri:noRegion>901 83 Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Moritz, Thomas" sort="Moritz, Thomas" uniqKey="Moritz T" first="Thomas" last="Moritz">Thomas Moritz</name><affiliation wicri:level="1"><nlm:affiliation>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå</wicri:regionArea><wicri:noRegion>901 83 Umeå</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>2The NovoNordisk Foundation Centre for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>2The NovoNordisk Foundation Centre for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen</wicri:regionArea><placeName><settlement type="city">Copenhague</settlement><region type="région" nuts="2">Hovedstaden</region></placeName></affiliation></author></analytic><series><title level="j">Plant methods</title><idno type="ISSN">1746-4811</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>Sugar phosphates are important intermediates of central carbon metabolism in biological systems, with roles in glycolysis, the pentose-phosphate pathway, tricarboxylic acid (TCA) cycle, and many other biosynthesis pathways. Understanding central carbon metabolism requires a simple, robust and comprehensive analytical method. However, sugar phosphates are notoriously difficult to analyze by traditional reversed phase liquid chromatography.</p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>Here, we show a two-step derivatization of sugar phosphates by methoxylamine and propionic acid anhydride after chloroform/methanol (3:7) extraction from </p></div><div type="abstract" xml:lang="en"><p><b>Conclusion</b></p><p>The results show the reliability of combined reversed phase liquid chromatography-tandem mass spectrometry for sugar phosphate analysis and demonstrate the presence of two unknown sugar phosphates in </p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31719834</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1746-4811</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Plant methods</Title><ISOAbbreviation>Plant Methods</ISOAbbreviation></Journal><ArticleTitle>Two-step derivatization for determination of sugar phosphates in plants by combined reversed phase chromatography/tandem mass spectrometry.</ArticleTitle><Pagination><MedlinePgn>127</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13007-019-0514-9</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Sugar phosphates are important intermediates of central carbon metabolism in biological systems, with roles in glycolysis, the pentose-phosphate pathway, tricarboxylic acid (TCA) cycle, and many other biosynthesis pathways. Understanding central carbon metabolism requires a simple, robust and comprehensive analytical method. However, sugar phosphates are notoriously difficult to analyze by traditional reversed phase liquid chromatography.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">Here, we show a two-step derivatization of sugar phosphates by methoxylamine and propionic acid anhydride after chloroform/methanol (3:7) extraction from <i>Populus</i> leaf and developing wood that improves separation, identification and quantification of sugar phosphates by ultra high performance liquid chromatography-electrospray ionization-mass spectrometry (UHPLC-ESI-MS). Standard curves of authentic sugar phosphates were generated for concentrations from pg to ng/μl with a correlation coefficient <i>R</i><sup>2</sup> > 0.99. The method showed high sensitivity and repeatability with relative standard deviation (RSD) < 20% based on repeated extraction, derivatization and detection. The analytical accuracy for <i>Populus</i> leaf extracts, determined by a two-level spiking approach of selected metabolites, was 79-107%.</AbstractText><AbstractText Label="Conclusion" NlmCategory="UNASSIGNED">The results show the reliability of combined reversed phase liquid chromatography-tandem mass spectrometry for sugar phosphate analysis and demonstrate the presence of two unknown sugar phosphates in <i>Populus</i> extracts.</AbstractText><CopyrightInformation>© The Author(s) 2019.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rende</LastName><ForeName>Umut</ForeName><Initials>U</Initials><AffiliationInfo><Affiliation>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden.</Affiliation><Identifier Source="ISNI">0000 0000 8578 2742</Identifier><Identifier Source="GRID">grid.6341.0</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Niittylä</LastName><ForeName>Totte</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden.</Affiliation><Identifier Source="ISNI">0000 0000 8578 2742</Identifier><Identifier Source="GRID">grid.6341.0</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moritz</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><Identifier Source="ORCID">0000-0002-4258-3190</Identifier><AffiliationInfo><Affiliation>1Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden.</Affiliation><Identifier Source="ISNI">0000 0000 8578 2742</Identifier><Identifier Source="GRID">grid.6341.0</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>2The NovoNordisk Foundation Centre for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.</Affiliation><Identifier Source="ISNI">0000 0001 0674 042X</Identifier><Identifier Source="GRID">grid.5254.6</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Methods</MedlineTA><NlmUniqueID>101245798</NlmUniqueID><ISSNLinking>1746-4811</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Extraction</Keyword><Keyword MajorTopicYN="N">Q-TOF</Keyword><Keyword MajorTopicYN="N">QqQ-MS</Keyword><Keyword MajorTopicYN="N">Sugar phosphates</Keyword><Keyword MajorTopicYN="N">Two-step derivatization</Keyword><Keyword MajorTopicYN="N">UHPLC–ESI–MS</Keyword></KeywordList><CoiStatement>Competing interestsThe authors declare that they have no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>05</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>10</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>11</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>11</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>14</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31719834</ArticleId><ArticleId IdType="doi">10.1186/s13007-019-0514-9</ArticleId><ArticleId IdType="pii">514</ArticleId><ArticleId IdType="pmc">PMC6836659</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biochem J. 2006 Jul 1;397(1):139-48</Citation><ArticleIdList><ArticleId IdType="pubmed">16551270</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chim Acta. 2011 Apr 29;692(1-2):1-25</Citation><ArticleIdList><ArticleId IdType="pubmed">21501708</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2008 Sep;55(6):1047-60</Citation><ArticleIdList><ArticleId IdType="pubmed">18494852</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2004 May 15;76(10):2869-77</Citation><ArticleIdList><ArticleId IdType="pubmed">15144199</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1964 Dec;9:431-42</Citation><ArticleIdList><ArticleId IdType="pubmed">14239480</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chromatogr A. 2006 Aug 25;1125(1):76-88</Citation><ArticleIdList><ArticleId IdType="pubmed">16759663</ArticleId></ArticleIdList></Reference><Reference><Citation>J Sep Sci. 2005 Sep;28(14):1823-30</Citation><ArticleIdList><ArticleId IdType="pubmed">16224979</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chromatogr A. 2007 Nov 23;1172(2):170-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17961583</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2018 Apr 3;90(7):4470-4477</Citation><ArticleIdList><ArticleId IdType="pubmed">29533656</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1993 Dec;215(2):243-52</Citation><ArticleIdList><ArticleId IdType="pubmed">8122785</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2009 Sep;59(5):826-39</Citation><ArticleIdList><ArticleId IdType="pubmed">19453453</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chim Acta. 2010 Sep 23;678(1):117-23</Citation><ArticleIdList><ArticleId IdType="pubmed">20869512</ArticleId></ArticleIdList></Reference><Reference><Citation>Rapid Commun Mass Spectrom. 2008 May;22(9):1399-407</Citation><ArticleIdList><ArticleId IdType="pubmed">18384194</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2013 Jun 18;85(12):5965-73</Citation><ArticleIdList><ArticleId IdType="pubmed">23682691</ArticleId></ArticleIdList></Reference><Reference><Citation>J Sep Sci. 2006 Aug;29(12):1784-821</Citation><ArticleIdList><ArticleId IdType="pubmed">16970185</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 2004 Aug 15;331(2):283-95</Citation><ArticleIdList><ArticleId IdType="pubmed">15265734</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chromatogr A. 2007 Apr 20;1147(2):153-64</Citation><ArticleIdList><ArticleId IdType="pubmed">17376459</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 1998 Sep;16(9):373-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9744112</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2017 Jul 18;89(14):7667-7674</Citation><ArticleIdList><ArticleId IdType="pubmed">28581703</ArticleId></ArticleIdList></Reference><Reference><Citation>Metab Eng. 2019 Jan;51:43-49</Citation><ArticleIdList><ArticleId IdType="pubmed">30176394</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2004 Feb 11;52(3):428-38</Citation><ArticleIdList><ArticleId IdType="pubmed">14759128</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1992 Sep;188(2):238-44</Citation><ArticleIdList><ArticleId IdType="pubmed">24178260</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2006 Sep 15;78(18):6573-82</Citation><ArticleIdList><ArticleId IdType="pubmed">16970336</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2003 Jun;6(3):236-46</Citation><ArticleIdList><ArticleId IdType="pubmed">12753973</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2009 Aug 1;81(15):6165-74</Citation><ArticleIdList><ArticleId IdType="pubmed">19522513</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 1978 Nov 14;85(1):257-63</Citation><ArticleIdList><ArticleId IdType="pubmed">743278</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Danemark</li><li>Suède</li></country><region><li>Hovedstaden</li></region><settlement><li>Copenhague</li></settlement></list><tree><country name="Suède"><noRegion><name sortKey="Rende, Umut" sort="Rende, Umut" uniqKey="Rende U" first="Umut" last="Rende">Umut Rende</name></noRegion><name sortKey="Moritz, Thomas" sort="Moritz, Thomas" uniqKey="Moritz T" first="Thomas" last="Moritz">Thomas Moritz</name><name sortKey="Niittyl, Totte" sort="Niittyl, Totte" uniqKey="Niittyl T" first="Totte" last="Niittyl">Totte Niittyl</name></country><country name="Danemark"><region name="Hovedstaden"><name sortKey="Moritz, Thomas" sort="Moritz, Thomas" uniqKey="Moritz T" first="Thomas" last="Moritz">Thomas Moritz</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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